3P54
Crystal Structure of the Japanese Encephalitis Virus Envelope Protein, strain SA-14-14-2.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-04 |
| Detector | MARMOSAIC 225 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 61.111, 62.398, 243.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.188 - 2.097 |
| R-factor | 0.1811 |
| Rwork | 0.179 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.098 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.097 | 4.520 | 2.097 |
| Rmerge | 0.070 | 0.031 | 0.427 |
| Number of reflections | 26920 | ||
| <I/σ(I)> | 16.4 | ||
| Completeness [%] | 96.9 | 98.7 | 96.9 |
| Redundancy | 4.3 | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 16% PEG 3350, 0.2M Sodium citrate, 0.1M Tris pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
