3P4Z
Time-dependent and Protein-directed In Situ Growth of Gold Nanoparticles in a Single Crystal of Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 123.2 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0988 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.584, 78.584, 37.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.1933 |
| Rwork | 0.193 |
| R-free | 0.23580 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.022 |
| Data reduction software | HKL-2000 |
| Phasing software | MOLREP (in the CCP4 Package) |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.157 |
| Number of reflections | 15391 |
| <I/σ(I)> | 29.1 |
| Completeness [%] | 97.5 |
| Redundancy | 25.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.5 | 298 | 6.5% NaCl (w/v) in 0.1 M sodium acetate, pH 4.5, EVAPORATION, temperature 298K |
