3P4O
Crystal Structure of H2-Kb in complex with the mutant NP205-LCMV-V3A epitope YTAKYPNL, an 8-mer modified peptide from the LCMV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.750, 85.050, 89.180 |
| Unit cell angles | 90.00, 111.33, 90.00 |
Refinement procedure
| Resolution | 61.694 - 2.300 |
| R-factor | 0.2053 |
| Rwork | 0.202 |
| R-free | 0.27460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vac |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.102 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.400 | |
| High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
| Rmerge | 0.096 | 0.030 | 0.517 |
| Rmeas | 0.032 | 0.555 | |
| Number of reflections | 41372 | 532 | 4911 |
| <I/σ(I)> | 17.87 | 54.2 | 4.1 |
| Completeness [%] | 99.8 | 98.3 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.2M ammonium acetate, 16-24% PEG 3350, 0.1M Na-cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 277K |
