3P47
Crystal structure of Entamoeba histolytica Serine acetyltransferase 1 in complex with L-cysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0337 |
| Spacegroup name | H 3 |
| Unit cell lengths | 110.047, 110.047, 64.002 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.780 |
| R-factor | 0.1935 |
| Rwork | 0.191 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.513 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 3.830 | 1.780 |
| Rmerge | 0.073 | 0.044 | 0.296 |
| Number of reflections | 26982 | ||
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 97.1 | 99.7 | 80.8 |
| Redundancy | 5.5 | 5.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | Peg 2000, NaCl, Glycerol, Tris, L-cysteine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
