3P3W

Structure of a dimeric GluA3 N-terminal domain (NTD) at 4.2 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2010-09-29
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	110.960, 127.920, 130.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.410 - 4.200
R-factor	0.3075
Rwork	0.306
R-free	0.33770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.822
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	91.197	55.413	4.430
High resolution limit [Å]	4.200	13.280	4.200
Rmerge		0.062	0.822
Rmeas		0.074	0.964
Rpim		0.040	0.494
Total number of observations		1594	7168
Number of reflections	13979
<I/σ(I)>	3.7	12.1	1.7
Completeness [%]	99.7	96.5	99.8
Redundancy	3.6	3.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.7	293	200mM ammonium phosphate, 20% PEG3350, pH 4.7, VAPOR DIFFUSION, temperature 293K