3P3W
Structure of a dimeric GluA3 N-terminal domain (NTD) at 4.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-09-29 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 110.960, 127.920, 130.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.410 - 4.200 |
| R-factor | 0.3075 |
| Rwork | 0.306 |
| R-free | 0.33770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.822 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 91.197 | 55.413 | 4.430 |
| High resolution limit [Å] | 4.200 | 13.280 | 4.200 |
| Rmerge | 0.062 | 0.822 | |
| Rmeas | 0.074 | 0.964 | |
| Rpim | 0.040 | 0.494 | |
| Total number of observations | 1594 | 7168 | |
| Number of reflections | 13979 | ||
| <I/σ(I)> | 3.7 | 12.1 | 1.7 |
| Completeness [%] | 99.7 | 96.5 | 99.8 |
| Redundancy | 3.6 | 3.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.7 | 293 | 200mM ammonium phosphate, 20% PEG3350, pH 4.7, VAPOR DIFFUSION, temperature 293K |
