3P3G
Crystal Structure of the Escherichia coli LpxC/LPC-009 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0001 |
| Spacegroup name | P 61 |
| Unit cell lengths | 106.725, 106.725, 52.684 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.595 - 1.650 |
| R-factor | 0.1562 |
| Rwork | 0.155 |
| R-free | 0.17710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.104 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 4.480 | 1.650 |
| Rmerge | 0.074 | 0.052 | 0.497 |
| Number of reflections | 41012 | ||
| <I/σ(I)> | 9.8 | ||
| Completeness [%] | 99.6 | 98.8 | 94.4 |
| Redundancy | 8.5 | 8.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1 M HEPES pH 7.5, 1.5 M LiSO4 and 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
