3P3D
Crystal structure of the Nup53 RRM domain from Pichia guilliermondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-24 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 62.507, 62.507, 55.117 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.460 - 2.350 |
| R-factor | 0.2447 |
| Rwork | 0.238 |
| R-free | 0.28760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Poly-Ala of 1WWH |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.420 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.620 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 5437 | |
| <I/σ(I)> | 21.1 | 6.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 21 | 21.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 294 | 100mM sodium acetate, 20% PEG 1500, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
