3P3B

CRYSTAL STRUCTURE OF Galacturonate DEHYDRATASE FROM GEOBACILLUS SP. COMPLEXED WITH D-TARTRATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	C 1 2 1
Unit cell lengths	158.859, 66.051, 75.664
Unit cell angles	90.00, 95.48, 90.00

Refinement procedure

Resolution	41.200 - 1.651
R-factor	0.2175
Rwork	0.216
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.063
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	41.200
High resolution limit [Å]	1.651
Number of reflections	87428
Completeness [%]	93.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	30% PEG Monomethyl ether 2000, 0.1M potassium thiocyanate , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K