3P38
Crystal structure of the NS1 effector domain W182A mutant from influenza A/Vietnam/1203/2004 (H5N1) virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-19 |
| Detector | RAYONIX MX-225 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 78.785, 78.785, 186.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.270 - 2.760 |
| R-factor | 0.233 |
| Rwork | 0.231 |
| R-free | 0.27070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p31 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT (BUSTER 2.11.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.400 | 2.950 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.076 | 0.365 |
| Number of reflections | 15754 | |
| <I/σ(I)> | 19.7 | 2.6 |
| Completeness [%] | 99.4 | 42.2 |
| Redundancy | 7.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.4 | 285 | 0.1M Bicine, 1.8 M MgCl2, pH 8.4, VAPOR DIFFUSION, SITTING DROP, temperature 285K |
