3P37
Polo-like kinase I Polo-box domain in complex with FDPPLHSpTA phosphopeptide from PBIP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Collection date | 2010-04-22 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.969, 88.658, 67.590 |
| Unit cell angles | 90.00, 113.48, 90.00 |
Refinement procedure
| Resolution | 62.020 - 2.380 |
| R-factor | 0.23509 |
| Rwork | 0.232 |
| R-free | 0.28838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1UMW (chain A) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.061 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 62.020 |
| High resolution limit [Å] | 2.380 |
| Number of reflections | 25445 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM MES, 30% PEG 400, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
