3P36
Polo-like kinase I Polo-box domain in complex with DPPLHSpTA phosphopeptide from PBIP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Collection date | 2010-04-22 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.931, 50.116, 58.730 |
| Unit cell angles | 90.00, 99.31, 90.00 |
Refinement procedure
| Resolution | 57.930 - 1.590 |
| R-factor | 0.19757 |
| Rwork | 0.195 |
| R-free | 0.25237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1UMW (chain A) |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.255 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 57.930 |
| High resolution limit [Å] | 1.590 |
| Number of reflections | 27603 |
| Completeness [%] | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 100mM Na/K phosphate, 0.2M NaCl, 10% PEG 8000, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
