3P32

Hydrolysis of GTP to GDP by an MCM-associated and MeaB- and MMAA-like G-protein from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97946
Spacegroup name	C 2 2 21
Unit cell lengths	65.860, 188.220, 66.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.427 - 1.900
R-factor	0.1998
Rwork	0.199
R-free	0.22260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mdo
RMSD bond length	0.015
RMSD bond angle	1.406
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.044	0.017	0.555
Number of reflections	32985	407	2409
<I/σ(I)>	25.16	63.3	3.5
Completeness [%]	99.8	94.7	99.4
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	289	22.0 mg/mL MytuD00200aA1 PW25862 against 48% PEG 200, 0.1 M Na/K phosphate pH 6.2, 0.2 M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 289K