3P2U
Crystal structure of PhnP in complex with orthovanadate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.761, 75.285, 82.423 |
| Unit cell angles | 90.00, 125.52, 90.00 |
Refinement procedure
| Resolution | 35.267 - 1.480 |
| R-factor | 0.1624 |
| Rwork | 0.161 |
| R-free | 0.18590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.235 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.300 |
| High resolution limit [Å] | 1.480 |
| Rmerge | 0.062 |
| Number of reflections | 109380 |
| <I/σ(I)> | 48.3 |
| Completeness [%] | 95.5 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 0.1 M MES, 7 % PEG 8000, pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
