3P22
Crystal structure of the ENE, a viral RNA stability element, in complex with A9 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.746, 50.869, 91.380 |
| Unit cell angles | 90.00, 125.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.501 |
| R-factor | 0.21913 |
| Rwork | 0.218 |
| R-free | 0.23343 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.318 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.977 |
| Number of reflections | 18693 | |
| <I/σ(I)> | 20.5 | 1 |
| Completeness [%] | 97.8 | 85.3 |
| Redundancy | 3.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 17% MPD, 0.09 M magnesium acetate, 0.05 M MES, pH 5.6 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 2 | ||||
| 3 |
