3P1X
Crystal structure of DRBM 2 domain of Interleukin enhancer-binding factor 3 from Homo sapiens, Northeast Structural Genomics Consortium Target HR4527E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-02 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.572, 24.384, 71.626 |
| Unit cell angles | 90.00, 124.84, 90.00 |
Refinement procedure
| Resolution | 29.390 - 1.900 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.25000 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.036 | 0.064 |
| Number of reflections | 12584 | |
| <I/σ(I)> | 33.9 | |
| Completeness [%] | 94.0 | 92.1 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | 0.1M Sodium chloride, 0.1 M Tris, pH (8, 24% (w/v) PEG 20K, microbatch under oil method, temperature 277K |
