3P1U
Crystal structure of a SusD homolog (BDI_0600) from Parabacteroides distasonis ATCC 8503 AT 2.05 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97916,0.97860 |
| Spacegroup name | P 6 |
| Unit cell lengths | 130.242, 130.242, 114.822 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.700 - 2.050 |
| R-factor | 0.244 |
| Rwork | 0.243 |
| R-free | 0.26500 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.370 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.701 | 2.100 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.252 | 0.965 |
| Number of reflections | 69272 | |
| <I/σ(I)> | 7.3 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.47 | 293 | 1.13M ammonium sulfate, 0.1M phosphate-citrate pH 4.47, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
