3P1T

Crystal structure of a putative aminotransferase (BPSL1724) from Burkholderia pseudomallei K96243 at 2.60 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-12
Detector	MAR MAR325
Wavelength(s)	0.91837,0.97936,0.97922
Spacegroup name	I 41 2 2
Unit cell lengths	167.550, 167.550, 290.534
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.650 - 2.600
R-factor	0.202
Rwork	0.200
R-free	0.23400
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.647	2.690
High resolution limit [Å]	2.600	2.600
Rmerge		1.144
Number of reflections	62018
<I/σ(I)>		1.6
Completeness [%]	97.4	93.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.25	277	0.20M lithium sulfate, 0.90M potassium sodium tartrate, 0.1M CHES pH 9.25, Additive, 0.005 M alpha ketoglutaric acid, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K