3P1I
Ligand binding domain of human ephrin type-B receptor 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-21 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97895 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.160, 102.160, 117.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.750 - 2.100 |
| R-factor | 0.18695 |
| Rwork | 0.184 |
| R-free | 0.23462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3etp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.377 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 37051 | |
| <I/σ(I)> | 20.048 | 3.04 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291.1 | 1.6 M AMMONIUM SULFATE, 5% ISOPROPANOL, 0.1 M BIS-TRIS,PH 8.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 291.1K |
