3P1B

Crystal structure of the native serine acetyltransferase 1 from Entamoeba histolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9737
Spacegroup name	H 3
Unit cell lengths	110.457, 110.457, 63.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.240 - 1.770
R-factor	0.2098
Rwork	0.208
R-free	0.25450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.919
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.770	3.810	1.770
Rmerge	0.082	0.034	0.543
Number of reflections	26288
<I/σ(I)>	8.4
Completeness [%]	93.1	100	69.7
Redundancy	5.2	5.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	Ammonium sulfate, NaCl, Glycerol, Tris, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K