3P14
Crystal structure of L-rhamnose isomerase with a novel high thermo-stability from Bacillus halodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Wavelength(s) | 1.23999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.240, 164.880, 92.010 |
| Unit cell angles | 90.00, 115.97, 90.00 |
Refinement procedure
| Resolution | 45.780 - 2.510 |
| R-factor | 0.18859 |
| Rwork | 0.185 |
| R-free | 0.25551 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.825 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.112 |
| Number of reflections | 76165 |
| Completeness [%] | 98.2 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 283 | 10% (w/v) PEG 8000, 0.1M HEPES pH 6.0, 0.2M sodium acetate , VAPOR DIFFUSION, HANGING DROP, temperature 283K |
