3P0T
Crystal structure of an HIT-like protein from mycobacterium paratuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-24 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 55.610, 55.610, 205.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.165 |
| Rwork | 0.163 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3LB5 modified with the CCP4 program CHAINSAW and truncated |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.462 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.362 |
| Number of reflections | 26228 | |
| <I/σ(I)> | 27.46 | 2.7 |
| Completeness [%] | 99.0 | 91.2 |
| Redundancy | 8.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | EBS JCSG+ SCREEN G11: 100MM BISTRIS PH 6.5, 2M AMMONIUM SULPHATE; MYPAA.00754.B.A1 PS00728 AT 48MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
