3P0S
Crystal structure of Bombyx mori densovirus 1 capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.2 |
| Spacegroup name | P 1 |
| Unit cell lengths | 245.400, 245.600, 245.700 |
| Unit cell angles | 59.98, 67.93, 72.27 |
Refinement procedure
| Resolution | 45.000 - 3.100 |
| R-factor | 0.2089 |
| Rwork | 0.209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
| Rmerge | 0.044 | 0.021 | 0.122 |
| Number of reflections | 789029 | ||
| <I/σ(I)> | 16.9 | ||
| Completeness [%] | 95.7 | 97.7 | 76.6 |
| Redundancy | 1.9 | 2 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 2% PEG8000, 10mM Tris-HCl, 30% glycerol, initial protein concentration in drop 5mg/ml, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
