3P0K

Structure of Baculovirus Sulfhydryl Oxidase Ac92

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97692
Spacegroup name	C 1 2 1
Unit cell lengths	55.765, 80.839, 65.053
Unit cell angles	90.00, 105.95, 90.00

Refinement procedure

Resolution	50.000 - 1.470
Rwork	0.208
R-free	0.23350
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	0.950
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.470	1.470
Number of reflections	46687
<I/σ(I)>	19.2	2.54
Completeness [%]	99.1	99.1
Redundancy	6.8	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.4	293	0.1 M acetic acid, 50 mM arginine-HCl, 750 mM NaCl, 5% ethanol, 24% PEG 5000-MME, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	4.4	293	0.1 M acetic acid, 50 mM arginine-HCl, 750 mM NaCl, 5% ethanol, 24% PEG 5000-MME, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K