3P0C
Nischarin PX-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.880, 64.780, 80.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.513 - 2.273 |
| R-factor | 0.1755 |
| Rwork | 0.174 |
| R-free | 0.21000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.925 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.880 | 2.400 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.090 | 0.215 |
| Number of reflections | 14541 | |
| <I/σ(I)> | 21.1 | 11.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.7 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | 11% PEG monomethyl ether 2000, 0.3M NaCl, 0.1M Tris, 0.3M trimetylamine n-oxide, 1mM benzene 1,2,4-trisphosphate, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
