3P0B
Thermus thermophilus family GH57 branching enzyme: crystal structure, mechanism of action and products formed
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-03 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 119.532, 119.532, 74.097 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.000 - 1.350 |
| R-factor | 0.17043 |
| Rwork | 0.169 |
| R-free | 0.18905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ufa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.462 | |
| Number of reflections | 116916 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 7.7 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% (w/v) PEG 3350, 8% (v/v) Tacsimate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
