3P0A
X-ray structure of pentameric HIV-1 CA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.794, 122.128, 149.304 |
| Unit cell angles | 74.41, 74.36, 81.48 |
Refinement procedure
| Resolution | 45.562 - 5.954 |
| R-factor | 0.2989 |
| Rwork | 0.297 |
| R-free | 0.31560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p05 |
| RMSD bond length | 0.029 |
| RMSD bond angle | 1.827 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 6.320 |
| High resolution limit [Å] | 5.950 | 5.950 |
| Number of reflections | 14213 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 99.4 | 98.6 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30-32% PEG 2000 MME, 100 mM Tris, 0.4 M sodium iodide, pH 7-8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
