3P02

Crystal structure of a protein of unknown function (BACOVA_00267) from Bacteroides ovatus at 1.55 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.86700,0.97925,0.97913
Spacegroup name	P 21 21 21
Unit cell lengths	51.920, 61.920, 96.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.466 - 1.550
R-factor	0.1659
Rwork	0.165
R-free	0.19000
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.020
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.466	29.466	1.590
High resolution limit [Å]	1.550	6.930	1.550
Rmerge		0.047	0.877
Total number of observations		2101	13680
Number of reflections	45435
<I/σ(I)>	8.2	18.1	1.6
Completeness [%]	99.4	98.7	98.8
Redundancy	4.1	3.5	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	19.50% polyethylene glycol 3350, 0.2570M lithium acetate, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K