3OZ6
Crystal structure of MapK from Cryptosporidium Parvum, cgd2_1960
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.298, 91.746, 119.985 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.680 - 2.370 |
| R-factor | 0.2321 |
| Rwork | 0.231 |
| R-free | 0.25970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.115 | 0.037 | 0.889 |
| Number of reflections | 33480 | ||
| <I/σ(I)> | 5.5 | 1.6 | |
| Completeness [%] | 99.9 | 99.4 | 99.2 |
| Redundancy | 7 | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG 3350 0.1 M MgAcetate 4 mM AMPPNP 4 mM MGCl2 4 mM TCEP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
