3OYY
Structure of Pseudomonas aeruginosa elongation factor P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-14 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97865 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.852, 51.986, 67.581 |
| Unit cell angles | 90.19, 99.52, 104.57 |
Refinement procedure
| Resolution | 19.810 - 1.750 |
| R-factor | 0.20679 |
| Rwork | 0.204 |
| R-free | 0.25930 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.764 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.810 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 46460 |
| Completeness [%] | 90.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4.5 | 298 | 1.35M KPhosphate, 0.15M NaPhosphate, 0.2M NaAcetate PH 4.5, hanging drop, temperature 298K |
| 1 | hanging drop | 4.5 | 298 | 1.35M KPhosphate, 0.15M NaPhosphate, 0.2M NaAcetate PH 4.5, hanging drop, temperature 298K |
