3OYX
Haloferax volcanii Malate Synthase Magnesium/Glyoxylate Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-21 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 155.023, 155.023, 141.787 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.510 |
| R-factor | 0.1948 |
| Rwork | 0.192 |
| R-free | 0.24760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.068 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 5.350 | 2.500 |
| Rmerge | 0.096 | 0.049 | 0.453 |
| Number of reflections | 22203 | ||
| <I/σ(I)> | 17.8 | ||
| Completeness [%] | 98.1 | 94.3 | 90 |
| Redundancy | 3.4 | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 295 | PEG 4500, glycerol, 2M KCl,MgCl2, pH 4.4, supplemented with 0.1M glyoxylate, 0.1M MgCl2, vapor diffusion, sitting drop, temperature 295K |
