3OYE
Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with magnesium and the INSTI Compound2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2010-06-08 |
Detector | ADSC |
Wavelength(s) | 0.98011 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 159.500, 159.500, 123.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.010 - 2.740 |
R-factor | 0.2053 |
Rwork | 0.204 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oyb |
RMSD bond length | 0.012 |
RMSD bond angle | 1.556 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.014 | 39.014 | 2.890 |
High resolution limit [Å] | 2.740 | 8.660 | 2.740 |
Rmerge | 0.066 | 0.862 | |
Total number of observations | 6210 | 28685 | |
Number of reflections | 41952 | ||
<I/σ(I)> | 6.1 | 12.2 | 1.6 |
Completeness [%] | 99.6 | 95.9 | 99.9 |
Redundancy | 4.7 | 4.4 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 1.35 M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50 mM Mes-NaOH, 1mM EDTA, pH 6.5, vapor diffusion, hanging drop, temperature 291K |