3OY8

Crystal structure of human galectin-1 in complex with lactobionic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	293
Detector technology	CCD
Collection date	2009-02-16
Detector	BRUKER SMART 6000
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	44.711, 58.570, 111.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.810 - 2.190
R-factor	0.17516
Rwork	0.173
R-free	0.22606
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.051
Data reduction software	SAINT
Data scaling software	SCALA
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.200
High resolution limit [Å]	2.190
Number of reflections	14888
<I/σ(I)>	10
Completeness [%]	94.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	293	4-8 microlitre drops consisting of equal volumes of protein solution (20 mM sodium potassium phosphate buffer, pH 7.0, and protein at concentration of 10 mg/mL) and reservoir solution (0.2 M ammonium sulphate, 25% w/v polyethylene glycol 4000, 0.1M sodium acetate trihydrate, pH 6.2), VAPOR DIFFUSION, HANGING DROP, temperature 293K