3OY7

Crystal structure of a virus encoded glycosyltransferase in complex with GDP-mannose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2009-01-01
Detector	MarCCD 300
Wavelength(s)	0.98
Spacegroup name	C 2 2 21
Unit cell lengths	154.248, 243.189, 67.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	130.190 - 2.730
R-factor	0.25854
Rwork	0.257
R-free	0.28965
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.840
High resolution limit [Å]	2.700	2.740
Rmerge	0.091	0.566
Number of reflections	33711
<I/σ(I)>	19.1	2.3
Completeness [%]	98.4	86.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	crystallization solution:100mM Tris-HCl pH7.0, 20% w/v PEG3350 soaking solution: 100mM Tris-HCl pH7.0, 20% w/v PEG3350, 10mM GDP-mannose , VAPOR DIFFUSION, HANGING DROP, temperature 298K