3OY2
Crystal structure of a putative glycosyltransferase from Paramecium bursaria Chlorella virus NY2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | MarCCD 300 |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 153.615, 246.941, 67.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 130.190 - 2.310 |
| R-factor | 0.23476 |
| Rwork | 0.233 |
| R-free | 0.26780 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.778 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.074 | 0.490 |
| Number of reflections | 56045 | |
| <I/σ(I)> | 4.2 | |
| Completeness [%] | 99.8 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100mM Tris-HCl pH7.0, 20% w/v PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
