3OXS
Crystal Structure of HLA A*02:07 Bound to HBV Core 18-27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-06-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.210, 79.060, 110.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.820 - 1.750 |
| R-factor | 0.17874 |
| Rwork | 0.177 |
| R-free | 0.20699 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hhh |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.404 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 52.830 | 3.290 | 1.760 |
| High resolution limit [Å] | 1.700 | 2.940 | 1.700 |
| Rmerge | 0.061 | 0.041 | 0.708 |
| Number of reflections | 57808 | ||
| <I/σ(I)> | 19.7 | 38.9 | 1.9 |
| Completeness [%] | 98.7 | 99.9 | 87.9 |
| Redundancy | 6.3 | 6.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 0.1M sodium acetate tridydrate pH 4.6, 8% w/v polyethylene 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
