3OXP
Structure of phosphotransferase enzyme II, A component from Yersinia pestis CO92 at 1.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.961070 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.906, 57.946, 78.742 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.670 - 1.200 |
| R-factor | 0.14843 |
| Rwork | 0.147 |
| R-free | 0.17861 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.718 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.059 | 0.660 |
| Number of reflections | 81918 | |
| <I/σ(I)> | 23.12 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 2.5 M NH4 Sulphate, 0.1M Bis-Tris propane, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
