3OXN
The crystal structure of a putative transcriptional regulator from Vibrio parahaemolyticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.316, 50.488, 108.116 |
| Unit cell angles | 86.19, 82.89, 86.74 |
Refinement procedure
| Resolution | 38.906 - 2.700 |
| R-factor | 0.2479 |
| Rwork | 0.245 |
| R-free | 0.28990 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.116 | 0.475 |
| Number of reflections | 38304 | |
| <I/σ(I)> | 8.7 | 1.9 |
| Completeness [%] | 92.5 | 71.3 |
| Redundancy | 3.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.8 M Ammonia Sulfate, 1 M MES pH6.5, 10 mM Cobalt Chloride hexahydrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
