3OX8
Crystal Structure of HLA A*02:03 Bound to HBV Core 18-27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-02-05 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.279, 68.269, 68.318 |
| Unit cell angles | 70.20, 84.40, 84.45 |
Refinement procedure
| Resolution | 64.120 - 2.160 |
| R-factor | 0.1848 |
| Rwork | 0.182 |
| R-free | 0.23409 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.313 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 64.120 | 3.240 | 2.220 |
| High resolution limit [Å] | 2.100 | 3.050 | 2.160 |
| Rmerge | 0.066 | 0.137 | 0.345 |
| Number of reflections | 68748 | ||
| <I/σ(I)> | 4.68 | 11.41 | 2.1 |
| Completeness [%] | 95.8 | 100 | 80.7 |
| Redundancy | 4 | 4.7 | 1.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1M HEPSE pH7.5, 1.4M sodium citrate tribasic dihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
