3OX6
Crystal Structure of the calcium sensor calcium-binding protein 1 (CaBP1)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97958, 0.97972, 1.01987 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.716, 68.716, 344.195 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.25436 |
| Rwork | 0.252 |
| R-free | 0.29528 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.556 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 38103 | |
| <I/σ(I)> | 7.1 | 1.6 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 1.9M (NH4)2SO4, 5-7% 1,6-hexanediol, 0.1M citrate/sodium citrate pH5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
