3OW8

Crystal Structure of the WD repeat-containing protein 61

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97934
Spacegroup name	P 1
Unit cell lengths	64.544, 72.922, 81.514
Unit cell angles	114.34, 96.44, 90.12

Refinement procedure

Resolution	30.000 - 2.300
R-factor	0.245
Rwork	0.244
R-free	0.27660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3frx
RMSD bond length	0.011
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.340
High resolution limit [Å]	2.300	6.240	2.300
Rmerge	0.106	0.046	0.865
Number of reflections	58481
<I/σ(I)>	9.89		1.05
Completeness [%]	97.8	97.7	96
Redundancy	1.9	2	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Mix 0.5uL precipitant (6% PEG20000, 8% PEG-MME550, 0.2M calcium acetate), 0.5uL protein stock solution (11 mg/mL protein), 0.2 uL additive (5% w/v polyvinylpyrrolidine K15), vapor diffusion, sitting drop, temperature 291K