Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.3779 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 84.237, 111.008, 258.867 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.630 - 3.780 |
| R-factor | 0.2822 |
| Rwork | 0.280 |
| R-free | 0.32350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.188 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 4.030 |
| High resolution limit [Å] | 3.780 | 7.690 | 3.780 |
| Rmerge | 0.084 | 0.042 | 0.467 |
| Number of reflections | 21143 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 96.8 | 99.9 | 84.9 |
| Redundancy | 3.7 | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 15%PEG1000, 360mM Lithium Citrate, 5% Glycerol, 5% Isopropanol, pH 6.5,, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
