3OVG
The crystal structure of an amidohydrolase from Mycoplasma synoviae with Zn ion bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 89.256, 89.186, 96.059 |
| Unit cell angles | 98.95, 92.89, 119.86 |
Refinement procedure
| Resolution | 43.747 - 2.059 |
| R-factor | 0.1743 |
| Rwork | 0.172 |
| R-free | 0.20970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3msr |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.417 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.130 |
| High resolution limit [Å] | 2.059 | 2.060 |
| Rmerge | 0.106 | 0.347 |
| Number of reflections | 152064 | |
| <I/σ(I)> | 4.9 | 4.9 |
| Completeness [%] | 98.1 | 96.3 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.4 M Sodium malonate pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
