3OV9

Structure of the Nucleoprotein from Rift Valley Fever Virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	93
Detector technology	CCD
Collection date	2010-03-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 6
Unit cell lengths	180.878, 180.878, 47.743
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.430 - 1.600
R-factor	0.22334
Rwork	0.222
R-free	0.25376
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ouo
RMSD bond length	0.006
RMSD bond angle	0.883
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	156.600	1.680
High resolution limit [Å]	1.600	1.600
Number of reflections	111025
Completeness [%]	94.0	93.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	200 mM MgNO3, 17%(w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K