3OV0
Structure of dodecaheme cytochrome c GSU1996
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-02 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.54980 |
| Spacegroup name | I 4 |
| Unit cell lengths | 150.900, 150.900, 76.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.29800 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 4.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.048 | 0.351 |
| Number of reflections | 26822 | |
| <I/σ(I)> | 18.4 | 2.1 |
| Completeness [%] | 96.7 | 91.5 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.5 M ammonium sulfate, 2.5 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
