3OUV
SeMet Derivative of L512M mutant of PASTA domain 3 of Mycobacterium tuberculosis PknB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Detector technology | CCD |
| Collection date | 2000-11-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1271 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 24.439, 73.413, 32.583 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.583 - 2.004 |
| R-factor | 0.1814 |
| Rwork | 0.176 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Refinement software | PHENIX ((phenix.refine: dev_243)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 3995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | 0.2 Lithium sulfate, 0.1 M Sodium acetate, 30% (v/v) MPD, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
