3OUQ
Structure of N-terminal hexaheme fragment of GSU1996
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-11 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.73774 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 63.000, 84.200, 100.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.670 - 2.600 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 3.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.089 | 0.421 |
| Number of reflections | 15711 | |
| <I/σ(I)> | 15.7 | 2.1 |
| Completeness [%] | 98.1 | 85.2 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 2.5 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
