3OUO
Structure of the Nucleoprotein from Rift Valley Fever Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-03-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 6 |
| Unit cell lengths | 175.500, 175.500, 47.417 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.650 - 2.300 |
| R-factor | 0.19941 |
| Rwork | 0.197 |
| R-free | 0.24296 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.013 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.440 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 37672 | |
| Completeness [%] | 100.0 | 98.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 200mM MgNO3, 17% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
