3OUN
Crystal structure of the FhaA FHA domain complexed with the intracellular domain of Rv3910
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115872 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 80.709, 80.709, 139.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.431 - 2.705 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.23980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3OTV and 2FEZ |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.114 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (- Phaser) |
| Refinement software | ELVES |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 14926 | |
| <I/σ(I)> | 6.3 | 1.77 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.2M CaCl2, 0.1M MES pH 6, 1mM MnCl2, 20% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
