3OUH

PHD2-R127 with JNJ41536014

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-03
Spacegroup name	P 63
Unit cell lengths	109.860, 109.860, 40.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.929 - 2.510
R-factor	0.208
Rwork	0.205
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.783
Refinement software	REFMAC (5.5.0104)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.650
High resolution limit [Å]	2.510	2.510
Rmerge		0.796
Number of reflections	9661
<I/σ(I)>	5.3	0.9
Completeness [%]	99.9	100
Redundancy	6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	2M AMMONIUM SULFATE/100MM IMIDAZOLE, 100 MM MAGNESIUM SULFATE/10% (V/V) GLYCEROL, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 293K