3OTR
2.75 Angstrom Crystal Structure of Enolase 1 from Toxoplasma gondii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.07809 |
| Spacegroup name | I 4 |
| Unit cell lengths | 323.632, 323.632, 66.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.550 - 2.750 |
| R-factor | 0.17325 |
| Rwork | 0.171 |
| R-free | 0.21881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pa6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.315 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.113 | 0.543 |
| Number of reflections | 90781 | |
| <I/σ(I)> | 11.6 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | Protein: 7.0 mG/mL, 0.25 Sodium cloride, 0.01M Tris-HCl pH 8.3; Screen: PACT (D3), 0.1M MMT buffer pH 6.0, 25% (w/v) PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
